Heritability of the resistance to pepper huasteco yellow vein virus in wild genotypes of <Emphasis Type="Italic">Capsicum annuum</Emphasis>

Pepper huasteco yellow vein virus (PHYVV) is the main virus of pepper crop in Mexico. No resistant cultivars are available and resistance breeding is hampered by the lack of knowledge of heritability (h ²) of PHYVV resistance. This is a continuation of previous studies and the objectives were to analyze the h ² and the behavior of the resistant trait to PHYVV. Four resistant assays were done with three resistant wild lines (UAS12, UAS13 and UAS10) of Capsicum annuum in the S4, S5, S6 and S7 generation under greenhouse conditions. Plants from all tests were inoculated with PHYVV through Bemisia tabaci. Line UAS12 was the most resistant showing a significantly proportion of resistant plants, less disease symptoms and longer incubation time, followed by the lines UAS13 and UAS10 in all assays. Distribution of symptoms showed a bimodal tendency in all the trials, suggesting that two groups of genes are involved in this resistance trait. The lines UAS12, UAS13 and UAS10 showed the same pattern of response to selection with an average of h ² of 0.17, 0.06, 0.02 and 0.00 in the S4, S5, S6 and S7, respectively. These results indicate that all lines responded positively to the selection in the S4, S5 and S6, whereas in the S7 there was no response by the possible exhaustion of variation. Line UAS12 is the most promising genotype and the lines UAS13 and UAS10 are genetic resources that can be supplemented to breed the resistance of PHYVV. These results provides basic information for resistance breeding.     

Environmental heterogeneity and biotic interactions as potential drivers of spatial patterning of shorebird nests

Context

Species distributions are driven by a wide variety of abiotic and biotic factors, including nest placement for breeding individuals. As such, the spatial distribution of nests within a landscape can reflect environmental heterogeneity, habitat preferences, or even interactions with predators and other species.

Objectives

We determined the extent to which environmental heterogeneity and predation risk accounted for the observed spatial distribution of nests.

Methods

We assessed the spatial distribution of 112 nests of a migratory shorebird, the Hudsonian Godwit (Limosa haemastica), at Beluga River, Alaska, from 2009 to 2012, and explicitly tested for the relative influence of habitat characteristics and predation risk on nest locations. We also evaluated the effect of nest location, distance to conspecific nests, and proximity to roads on nest fate using 64 nests that were monitored through completion.

Results

Hudsonian Godwit nests were clustered across the landscape despite a lack of significant spatial autocorrelation (i.e., patchiness) in vegetation characteristics at either the micro- or landscape scale. Nest fate also was not predicted by either the distance to the nearest conspecific neighbor or proximity to roads. Thus, neither habitat characteristics nor predation risk explained the clustering of godwit nests.

Conclusions

These results suggest that godwits may select nest locations based more on social cues than underlying heterogeneity in vegetation or predation risk. As such, intra- and inter-specific interactions should be considered when developing management plans for species of conservation concern.

     

A rapid assessment of landscape biodiversity using diversity profiles of arthropod morphospecies

Context

The assessment of land-use impacts on biodiversity is one of the central themes of landscape ecology and conservation biology. However, due to the complexity of biodiversity, it is impossible to obtain complete information about the diversity of all species even for small areas, necessitating the selection of individual species or assemblages thereof as species surrogate. In parts of the world where taxonomic expertise is lacking, species identification has hindered progress in biodiversity conservation, and the only practical, relatively-accurate option, is the use of taxonomic minimalism.

Objective

We carried out a rapid biodiversity assessment based on three surrogates—land-use (driver-surrogate), terrestrial arthropods (species-surrogate) and morphospecies (taxonomic-surrogate)—to determine the impacts of land-use on biodiversity of the Western Region (Ghana), an area covering ~4 % of the West African biodiversity hotspot.

Method

We used diversity profiles to visualize the distribution of a total of 8848 arthropod individuals over seven land-use types which define the complete heterogeneity of the landscape.

Results

Here, we present both sample and asymptotic diversity profiles of arthropod morphospecies for each land-use type and the potential of each land-use type for conserving arthropods.

Conclusions

We conclude that (1) the morphospecies approach is useful for detecting differences in species diversity of land-use types; (2) the concept of asymptotic diversity may not be necessary for land-use based biodiversity comparison; and (3) maximum diversity profiles are useful for determining the land-use conservation values in cases where pristine areas are not available.

     

Sex‐dependent responses of perch to changes in water clarity and temperature

Rising temperatures and decreasing water transparency of lakes have strong wide ranging effects on fish. Fish responses to various changes in the environment are usually species‐dependent, but responses may also vary within species. In general, large individuals are considered to be more sensitive to environmental variation due to higher energy demand, than smaller individuals. Similarly, large individuals require more food to maintain bodily functions and are thus more sensitive to resource and food scarcity. These size‐specific responses to environmental gradients are also sex‐dependent in species that exhibit sexual size dimorphism (SSD). We studied in enclosures with short‐term experiments how rising temperatures and decreasing water transparency regulate the feeding rates of female and male European perch (Perca fluviatilis L.). To explore experimental results, we calculated perch SSD in nine lakes with varying environmental conditions using previously collected field data. The results of the experiments revealed that the combined effect of water transparency and temperature on the feeding rate of fish is gender‐dependent: feeding rate of females decreased more than that of males. The experimental results were also supported by field data that revealed a negative relation between water transparency and the magnitude of SSD in perch. Our results suggest that rising temperatures and decreasing water transparency may potentially decrease fish size in a sex‐dependent manner. As female size is one of the main demographic traits determining the reproductive success of a fish population, changing environments may have unexpected and far‐reaching consequences on fish population dynamics.     

A network model framework for prioritizing wetland conservation in the Great Plains

Context

Playa wetlands are the primary habitat for numerous wetland-dependent species in the Southern Great Plains of North America. Plant and wildlife populations that inhabit these wetlands are reciprocally linked through the dispersal of individuals, propagules and ultimately genes among local populations.

Objective

To develop and implement a framework using network models for conceptualizing, representing and analyzing potential biological flows among 48,981 spatially discrete playa wetlands in the Southern Great Plains.

Methods

We examined changes in connectivity patterns and assessed the relative importance of wetlands to maintaining these patterns by targeting wetlands for removal based on network centrality metrics weighted by estimates of habitat quality and probability of inundation.

Results

We identified several distinct, broad-scale sub networks and phase transitions among playa wetlands in the Southern Plains. In particular, for organisms that can disperse >2 km a dense and expansive wetland sub network emerges in the Southern High Plains. This network was characterized by localized, densely connected wetland clusters at link distances (h) >2 km but <5 km and was most sensitive to changes in wetland availability (p) and configuration when h = 4 km, and p = 0.2–0.4. It transitioned to a single, large connected wetland system at broader spatial scales even when the proportion of inundated wetland was relatively low (p = 0.2).

Conclusions

Our findings suggest that redundancy in the potential for broad and fine-scale movements insulates this system from damage and facilitates system-wide connectivity among populations with different dispersal capacities.

     

Establishment of a comprehensive indicator system for the assessment of biodiversity and ecosystem services

Context

Common indicators are needed to monitor biodiversity loss and the implications for the sustainable provision of ecosystem services (ES). A plethora of different sets of indicators may hinder the identification of major endpoints for large-scale assessments of biodiversity and ecosystem services (BES).

Objectives

We aim to describe the main challenges of indicators for BES assessment and provide suggestions for establishing a comprehensive indicator system.

Methods

An extensive literature review was conducted in this study. We review the main challenges of indicators for BES assessment and propose corresponding improvements from our perspectives of theory and practical applications.

Results

The main theoretical challenges of existing indicators include inconsistent definitions and classifications of ES, misunderstanding of linkages among biodiversity, ecosystem structure, functions and services, and practical problems relate to such issues as indicator representativeness, data availability, and uncertainty. Our suggested improvements include clarifying the main terms, concepts and classification of indicators, establishing a robust conceptual framework with clear interactions among different components and indicators, selecting indicators based on ecosystem properties, streaming existing data into one platform, and strengthening validation of proxies. The steps for constructing a comprehensive indicator system for BES assessment are summarized.

Conclusions

Clear definitions of key terms are indispensable to classify indicators and construct a conceptual framework. Improved understanding of the relations among indicators of biodiversity, ecosystem functions, and ES across multiple scales can guide the development of the indicator system. The integrated indicator system is an important tool for BES assessment to support decision making for sustainable development.

     

Pedotransfer functions to assess adsorbed phosphate using iron oxide content and magnetic susceptibility in an Oxisol

Adsorbed phosphate in soils can be chemically extracted; however, this process is both time‐consuming and not cost‐effective if large numbers of samples have to be analysed. Indirect assessment of adsorbed phosphate by pedotransfer functions (PTFs) can help optimize fertilizer strategies. This study aimed to evaluate the spatial variability of adsorbed phosphate (P_ads), iron oxides and magnetic susceptibility (MS) in oxisols and to calibrate PTFs to predict P_ads. A total of 308 soil samples were collected from Hapludox and Eutrudox soils formed from sandstone in Brazil. The contents of clay (196–607 g/kg), iron oxides (40–165 g/kg), MS (1.2–29 × 10^?6 m³/kg) and P_ads (327–842 mg/kg) were in the range of typical values for these highly weathered soils. This study showed that the attributes studied were spatially dependent. Geomorphic surfaces enabled understanding of spatial variability and helped to develop a more efficient sampling scheme to calibrate PTFs. Moreover, the adsorbed phosphate in these oxisols could be predicted by a PTF using iron oxides and MS as predictors. The MS attribute enabled the most accurate prediction (concordance coefficient = 0.95, root‐mean‐square error = 46 mg/kg and relative improvement in root‐mean‐square error = ?4.12) of spatial variability through PTF compared to other predictors.     

Symbiotic N<Subscript>2</Subscript> fixation and nitrate utilisation in irrigated lucerne (<Emphasis Type="Italic">Medicago sativa</Emphasis>) systems

Symbiotic N₂ fixation by lucerne (Medicago sativa) has capacity to provide significant inputs of N to agro-ecosystems, and the species has also been shown to scavenge soil mineral N and thus act as a sink for excess reactive N. The balance between these two N cycle processes was investigated in an extensive irrigated lucerne growing region where nitrate contamination of groundwater has been reported. We sampled 18 permanent pure lucerne stands under irrigation for standing dry matter, total shoot N, and N₂ fixation using ¹⁵N natural abundance along with activity of the inducible enzyme nitrate reductase as indicators of use of soil NO₃⁻ by lucerne. On average 65% of lucerne N was obtained from symbiotic N₂ fixation. Converting standing dry matter estimates to annual N₂ fixation amounts we calculated average N₂ fixation of 311 kg N/ha, including N in roots and nodules. Uptake of N from soil by lucerne was calculated to be 181 kg N/ha/year. We were not able to identify the source of this soil mineral N, although nitrate reductase activity of lucerne was higher than that of non-N₂ fixing species examined.     

Characterization of resistance to the wheat stem sawfly in spring wheat landrace accessions from targeted geographic regions of the world

Plant landraces have long been recognized as potential gene pools for biotic and abiotic stress-related genes. This research used spring wheat landrace accessions to identify new sources of resistance to the wheat stem sawfly (WSS) (Cephus cinctus Norton), an important insect pest of wheat in the northern Great Plains of North America. Screening efforts targeted 1409 accessions from six geographical areas of the world where other species of grain sawflies are endemic or where a high frequency of accessions possesses the resistance characteristic of solid stems. Resistance was observed in approximately 14% of accessions. Half of the lines displayed both antixenosis and antibiosis types of resistance. Among the resistant accessions, 41% had solid or semi-solid stems. Molecular genetic screening for haplotypes at the solid stem QTL, Qss.msub.3BL, showed that 15% of lines shared the haplotype derived from ‘S-615’, the original donor of the solid stem trait to North American germplasm. Other haplotypes associated with solid stems were also observed. Haplotype diversity was greater in the center of origin of wheat. Evaluation of a representative set of resistant landrace accessions in replicated field trials at four locations over a three year period identified accessions with potential genes for reduced WSS infestation, increased WSS mortality, and increased indirect defense via parasitoids. Exploitation of distinct types of plant defense will expand the genetic diversity for WSS resistance currently present in elite breeding lines.